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PROMOTING FEMALE HEALTH AT EVERY STAGE

What is Vitrification?

Vitrification is an advanced cryopreservation technique used to preserve sperm, oocytes (eggs), and embryos at extremely low temperatures. Unlike traditional slow freezing, vitrification is a rapid freezing process that turns the cells into a glass-like solid state without forming ice crystals—preventing cellular damage and ensuring high survival rates upon thawing.

At Sriram Hospitals, we utilize state-of-the-art vitrification methods to offer reliable fertility preservation options to couples and individuals undergoing fertility treatments or those planning to delay parenthood.

Liquid nitrogen tank used for embryo freezing
Doctor handling cryogenic storage tank for embryos

The Science Behind Vitrification

In traditional freezing, slow cooling allows water inside the cells to crystallize, which can damage delicate structures like the spindle apparatus in eggs or the cellular membranes in embryos.

Vitrification avoids this by:

  1. Using Cryoprotectants – Special protective solutions replace intracellular water to prevent crystal formation.

  2. Ultra-Rapid Cooling – The specimens are plunged into liquid nitrogen at -196°C within seconds, instantly transforming water into a stable glass-like state without crystal formation.

  3. Maintaining Cellular Integrity – This process preserves both the physical structure and the genetic material of the cells.

Why Choose Vitrification?

Vitrification is an essential tool for both medical and personal reasons. It is recommended for:

  • Fertility Preservation Before Medical Treatment
    Patients undergoing cancer treatments like chemotherapy or radiation, which may damage reproductive cells, can preserve their eggs, sperm, or embryos beforehand.

  • Elective Fertility Preservation
    Women who wish to delay childbearing for career, education, or personal reasons can preserve their eggs while they are at their healthiest stage.

  • IVF Cycle Management
    Couples undergoing IVF can freeze excess high-quality embryos for use in later cycles, avoiding the need for repeated stimulation and egg retrieval.

  • Donor Programs
    Donor eggs and sperm are often vitrified to maintain quality until they are matched with recipients.

  • Medical Conditions
    Certain genetic or hormonal conditions that may affect fertility can be managed by preserving reproductive cells early.

Here are some of the check up and health screenings that you should get done:

  • Pelvic Exam

  • Pap Smear (Cervical Cancer Screening)

  • Breast Exam

  • Blood Pressure Measurement

  • Cholesterol Screening

  • Bone Density Scan (DXA)

  • Blood Sugar Test (Glucose Screening)

  • Thyroid Function Tests

  • Blood Count (Complete Blood Count, CBC)

Step-by-Step Process at Sriram Hospitals

  • Patient Consultation & Planning
    Our fertility specialists assess your medical history, fertility goals, and the best approach for preservation.

  • Collection of Specimens

    • For Eggs – Ovarian stimulation is done to produce multiple mature eggs, followed by egg retrieval.

    • For Sperm – Collected through ejaculation or surgical sperm retrieval.

    • For Embryos – Created in the IVF lab by fertilizing eggs with sperm before freezing.

  • Preparation for Freezing
    The cells are treated with cryoprotectants to replace water content and protect against freezing damage.

  • Vitrification
    Using specialized tools, the eggs, sperm, or embryos are cooled instantly to -196°C and stored in liquid nitrogen tanks.

  • Storage & Monitoring
    Our cryo-storage facility is equipped with advanced monitoring systems to ensure constant temperature and safety.

  • Thawing & Use
    When you are ready to use your preserved cells, they are warmed rapidly and prepared for IVF or ICSI procedures.

Conclusion

Whether you are preserving your fertility for personal reasons, preparing for medical treatment, or managing your IVF cycles, vitrification offers a reliable, safe, and highly effective way to protect your reproductive potential. At Sriram Hospitals, we are committed to offering world-class care, advanced technology, and unwavering support throughout your fertility journey.

Vitrification is not just a scientific process; it’s a deeply personal choice. For many, it means peace of mind, the freedom to focus on other life priorities, or the chance to protect fertility before facing a medical challenge. At Sriram Hospitals, we combine medical excellence with compassionate guidance to make the process as smooth and stress-free as possible.

Cryotube with embryos in liquid nitrogen freezing

Vitrification 

Empowering Women's Health

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